Memphis Depay has missed nine of his 36 penalties including shootouts
Memphis Depay missed a penalty as Barcelona lost at Rayo Vallecano to increase the pressure on Ronald Koeman.
Barca, defeated 2-1 at home to Real Madrid on Sunday, are ninth in La Liga.
Oscar Trejo won the ball from Sergio Busquets and fed veteran striker Radamel Falcao, who finished via the inside of the post for the only goal.
The visitors won a penalty when Oscar Valentin fouled Depay but his spot-kick was well saved by Stole Dimitrievski, who also smothered the rebound well.
"It [Barcelona's league position] says we're not well," said Barca boss Koeman.
"The team has lost balance in the squad, lost very effective players, which shows. In recent years other clubs have strengthened every season and we haven't, which also shows."
Asked if the defeat could affect his future, he said: "I don't know."
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It is the first time since September 1987 that Barca have lost three away games in a row without scoring - a run that saw English manager Terry Venables sacked.
Koeman - whose car was surrounded by angry fans after losing El Clasico - admitted before this game his side "cannot afford back-to-back losses".
Trejo, perhaps sensing Barcelona's fragile mental state, tried to score from the halfway line in the third minute but his shot was saved by Marc-Andre ter Stegen.
Barca were uninspired yet again, with Depay's penalty their only shot on target.
Luuk de Jong's chip, Sergio Aguero's header and Gavi's scuffed shot were off target late on.
This is their fifth defeat of the season in all competitions and they sit six points behind four-way leaders Real Madrid, Sevilla, Real Betis and Real Sociedad.
Real drew 0-0 with Osasuna in Wednesday's late game.
Rayo - who have won all five home games this season - rise to fifth, above city rivals and champions Atletico Madrid.
- Line-upsMatch StatsLive Text
Line-upsRayo Vallecano
Formation 4-2-3-1
13Dimitrievski20Balliu24Saveljich5Catena33García Torres23Valentín6Comesaña7Palazón8Trejo18García Rivera3Falcao
- 13Dimitrievski20Balliu24SaveljichBooked at 83mins5Catena33García Torres23ValentínSubstituted forSuárezat 90+2'minutes6ComesañaSubstituted forLópezat 63'minutes7Palazón8TrejoBooked at 24minsSubstituted forCissat 76'minutes18García Rivera3FalcaoSubstituted forNtekaat 76'minutes
Substitutes
- 1Zidane2Maras4Suárez9Nteka10Dias Correia11Martín12López16Guardiola Navarro19Rodrigues21Ciss22Pozo32Hernández
Barcelona
Formation 4-3-3
1ter Stegen20Roberto3Piqué24García18Alba28González Iglesias5Busquets14Coutinho2Dest19Agüero9Depay
- 1ter Stegen20RobertoSubstituted forDemirat 60'minutes3PiquéBooked at 66mins24García18Alba28González IglesiasSubstituted forde Jongat 84'minutes5Busquets14CoutinhoBooked at 52minsSubstituted forPaez Gaviriaat 74'minutes2Dest19Agüero9Depay
Substitutes
- 6Puig Martí11Demir13Murara Neto15Lenglet17de Jong22Mingueza23Umtiti26Peña Sotorres30Paez Gaviria31Balde Martínez
Referee:Antonio Miguel Mateu LahozAttendance:9,340Match StatsHome TeamRayo VallecanoAway TeamBarcelonaPossessionHome31%Away69%ShotsHome13Away16Shots on TargetHome3Away1CornersHome3Away7FoulsHome17Away12Live TextPost update
Match ends, Rayo Vallecano 1, Barcelona 0.
90'+9'Full Time
Second Half ends, Rayo Vallecano 1, Barcelona 0.
90'+8'Post update
Attempt missed. Gavi (Barcelona) right footed shot from the centre of the box is just a bit too high. Assisted by Sergio Agüero with a cross.
90'+7'Post update
Offside, Rayo Vallecano. Stole Dimitrievski tries a through ball, but Álvaro García is caught offside.
90'+7'Post update
Attempt missed. Sergio Agüero (Barcelona) header from the right side of the six yard box is close, but misses to the right. Assisted by Sergiño Dest.
90'+5'Post update
Attempt blocked. Randy Nteka (Rayo Vallecano) left footed shot from outside the box is blocked. Assisted by Mario Suárez.
90'+5'Post update
Attempt missed. Alejandro Catena (Rayo Vallecano) header from the centre of the box is just a bit too high. Assisted by Unai López with a cross following a corner.
90'+4'Post update
Corner, Rayo Vallecano. Conceded by Sergiño Dest.
90'+3'Post update
Memphis Depay (Barcelona) wins a free kick in the defensive half.
90'+3'Post update
Foul by Iván Balliu (Rayo Vallecano).
90'+2'Substitution
Substitution, Rayo Vallecano. Mario Suárez replaces Óscar Valentín.
90'+2'Post update
Attempt missed. Luuk de Jong (Barcelona) right footed shot from a difficult angle and long range on the right is too high. Assisted by Sergio Busquets.
90'Post update
Attempt blocked. Yusuf Demir (Barcelona) left footed shot from the centre of the box is blocked.
89'Post update
Attempt missed. Esteban Saveljich (Rayo Vallecano) header from very close range is just a bit too high. Assisted by Unai López with a cross following a corner.
89'Post update
Corner, Rayo Vallecano. Conceded by Jordi Alba.
85'Post update
Attempt blocked. Isi Palazón (Rayo Vallecano) left footed shot from the centre of the box is blocked. Assisted by Fran García.
84'Post update
Foul by Memphis Depay (Barcelona).
84'Post update
Iván Balliu (Rayo Vallecano) wins a free kick in the defensive half.
84'Substitution
Substitution, Barcelona. Luuk de Jong replaces Nicolás González Iglesias.
83'Booking
Esteban Saveljich (Rayo Vallecano) is shown the yellow card.
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Source: bbc.com