The Black Maidens were defeated 2-1 by Cameroon in their second friendly game in Brazil.
Ghana u-17 female national team had earlier thrashed the Brazilian female side America FC 4-0 but failed to beat their West Africa neighbours.
Despite dominating possession, Ghana had nothing to show off as they conceded twice before pulling a consolation midway in the second half.
Coach Evans Adotey and his charges are expected to play their final warm-up game before leaving for Uruguay for the upcoming FIFA U-17 Women’s World Cup.
Full squad: Goalkeepers: Grace Banwa Buoadu, Barikisu Issahaku, Cynthia Findib
Defenders: Nina Norshie, Mavis Owusu, Diana Antwi, Justice Tweneboah, Elizabeth Oppong, Selina Kurug Bane, Tedinah Sekyere, Faustina Aidoo
Midfielders: Jacqueline Owusu, Elshadai Acheampong, Azuma Bugre, Grace Animah, Basira Alhassan, Fuseina Mumuni
Forwards: Suzzy Dede Teye, Abigail Tutuwaa, Milot Abena Pokuaa, Mukarama Abdulai.